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Anti-EGFP sepharose Purification kit

Datasheet

Anti-EGFP sepharose, is consist of an EGFP VHH coupled to NHS-sepharose.
It is an efficient technique for isolating recombinant proteins or mammalian expression proteins.
The EGFP epitope system relies on a VHH EGFP recombinant antibody, which is able to react with N- and C- terminal GFP tagged and EGFP tagged fusion proteins.

 
  • This product can be used for the immuneprecipitation or immune affinity purification.
  • The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry and this kit allows a rapid and efficient affinity purification of active EGFP fusion proteins.
  • The affinity resin allows an efficient binding of EGFP fusion proteins without the need for preliminary steps and calibrations.
  • The affinity bound EGFP fusion proteins can be efficiently eluted from the resin by acid condition.
  • The eluted proteins can be used for characteristic analysis.
No. Size Price Qty Status
C07009-K01 5 rxns $400.00 Inquiry
C07009-K02 10 rxns $640.00 Inquiry
The price does not include shipping fee and tax. Order Request Quote
Component:
Reagent Quantity
for 10 rxns
(C07009-K02)		 Quantity
for 5 rxns
(C07009-K01)		 composition
Anti-EGFP
sepharose		 2 mL X 1 vial 1 mL X 1 vial 50% slurry of Anti-EGFP
sepharose in 20% ethanol
Wash Buffer (10X
concentration)		 5.0 mL X 1 vial 2.5 mL X 1 vial 10 mM Tris/Cl pH 7.5;
150 mM NaCl;
0.5 mM EDTA
Elution Buffer 10 mL X 1 vial 5 mL X 1 vial 200 mM Glycine pH 2.5
Neutralization Buffer 2 mL X 1 vial 1 mL X 1 vial 2 M Tris pH 8.0
spin column 10 pcs 5 pcs  
collection tube 10 tubes 5 tubes  
Note: Sepharose is 1:1 suspension in 20% ethanol as a preservative.
 
Product capacity:
The binding and elution capacity of 1 mL settled Anti-EGFP sepharose may be vary, commonly more than 1 mg GFP or EGFP fusion proteins. Trying different elution buffers for optimal results is recommended.
 
Materials Required but Not Provided:
  • Micro-centrifuge capable of 15,000 x g
  • 1.5 mL Centrifuge tubes
  • End-over-end rotator
  • CoIP Lysis Buffer (mild reaction): 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.5% NP-40
  • RIPA (vigorous reaction): 100 mM Tris/Cl pH 7.5; 300 mM NaCl; 0.2% Sodium Deoxycholate (or 0.1% SDS); 2% NP-40
  • 2 x SDS Loading Buffer without DTT & 2 x SDS Loading Buffer with 1 mM DTT
  • Pre-urea Buffer: 50 mM Tris pH 8.5; 1mM EGTA; 75 mM KCl
  • Urea Elution Buffer: 6-8 M urea; 20 mM Tris pH 7.5; 100 mM NaCl
Storage:
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.

Shipping Conditions:
Blue ice
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